α-galectin-3 antibody  (Galectin Therapeutics)

Journal: International Journal of Stem Cells

Article Title: Mesenchymal Stem Cells Ameliorate Fibrosis by Enhancing Autophagy via Inhibiting Galectin-3/Akt/mTOR Pathway and by Alleviating the EMT via Inhibiting Galectin-3/Akt/GSK3β/Snail Pathway in NRK-52E Fibrosis

doi: 10.15283/ijsc22014

Figure Lengend Snippet: Effects of SF-MSCs-CM on TGF-β-induced NRK-52E fibrosis at different time points. (A) The expression of α-SMA and Gal-3 in NRK-52E cells induced by TGF-β1 was detected by Western Blot after SF-MSCs-CM treatment. (B) Quantification of α-SMA. (C) Quantification of Gal-3. Results were normalized relative to the expression of GAPDH. N=3 (per group). Data are presented as mean±SD and analyzed by two-way ANOVA followed by Tukey post hoc testing. *p<0.05, vs. control group, # p<0.05, vs. TGF-β1＋SF-MSCs-CM (24 h) group, ● p<0.05, vs. TGF-β1＋SF-MSCs-CM (48 h) group, ▲ p<0.05, vs. TGF-β1 ＋SF-DMEM (24 h) group, ◆ p<0.05, vs. TGF-β1＋SF-DMEM (48 h) group.

Article Snippet: The following antibodies are used: GAPDH (1:30,000, Proteintech, USA), α-SMA (1:4,000, Proteintech, USA), Galectin-3 (1:3,000, Cell Signaling, USA), GSK3β (1:1,000, Cell Signaling, USA), Phospho-GSK3β (Ser9) (1:1,000, Cell Signaling, USA), Snail (C15D3) (1:1,000, Cell Signaling, USA), Akt (pan) (1:1,000, Cell Signaling, USA), Phospho-Akt (Ser473) (1:1,000, Cell Signaling, USA), Fibronectin (1:1,000, Abcam, UK), E-Cadherin (1:1,000, Abcam, UK), MMP9 (1:1,000, Abcam, UK), TIMP1 (1:1,000, Cell Signaling, USA), Kim-1 (1:1,000, SAB, USA), PI3K (1:1,000, Abmart, China), Phospho-PI3K (1:1,000, Abmart, China), mTOR (1:1,000, Abmart, China), Phospho-mTOR (1:1,000, Abmart, China), LC3B (1:1,000, Abmart, China), and P62 (1:1,000, Abmart, China) antibodies.

Techniques: Expressing, Western Blot, Control

Journal: International Journal of Stem Cells

Article Title: Mesenchymal Stem Cells Ameliorate Fibrosis by Enhancing Autophagy via Inhibiting Galectin-3/Akt/mTOR Pathway and by Alleviating the EMT via Inhibiting Galectin-3/Akt/GSK3β/Snail Pathway in NRK-52E Fibrosis

doi: 10.15283/ijsc22014

Figure Lengend Snippet: Immunocytochemical staining of E-Cadherin and α-SMA in Gal-3 KD and Gal-3 OE NRK-52E cells. Gal-3 KD reduced the expre-ssion of α-SMA and increased the expression of E-Cadherin in NRK-52E cells, but an opposite trend appea-red in Gal-3 OE. TGF-β1 indeed increased the expression of α-SMA and decreased the expression of E-Cadherin in Gal-3 OE cell, which was more than in Gal-3 KD cells. The treatment of SF-MSCs-CM after TGF-β1 treatment reduced the expression of α-SMA and raised the expression of E-Cadherin in both Gal-3 KD cells and Gal-3 OE cells, but more obvious in Gal-3 KD cells than in Gal-3 OE cells. SF-DMEM treatment also downregulated the expression of α-SMA, worse than SF-MSCs-CM group. Bar=100 μm.

Article Snippet: The following antibodies are used: GAPDH (1:30,000, Proteintech, USA), α-SMA (1:4,000, Proteintech, USA), Galectin-3 (1:3,000, Cell Signaling, USA), GSK3β (1:1,000, Cell Signaling, USA), Phospho-GSK3β (Ser9) (1:1,000, Cell Signaling, USA), Snail (C15D3) (1:1,000, Cell Signaling, USA), Akt (pan) (1:1,000, Cell Signaling, USA), Phospho-Akt (Ser473) (1:1,000, Cell Signaling, USA), Fibronectin (1:1,000, Abcam, UK), E-Cadherin (1:1,000, Abcam, UK), MMP9 (1:1,000, Abcam, UK), TIMP1 (1:1,000, Cell Signaling, USA), Kim-1 (1:1,000, SAB, USA), PI3K (1:1,000, Abmart, China), Phospho-PI3K (1:1,000, Abmart, China), mTOR (1:1,000, Abmart, China), Phospho-mTOR (1:1,000, Abmart, China), LC3B (1:1,000, Abmart, China), and P62 (1:1,000, Abmart, China) antibodies.

Techniques: Staining, Expressing